Vytrus Biotech unveils Capilia Longa – the hair 3D Bio-printer

Hair growth Re-Activation by genuine Plant Growth Factors

• Epigenetic Reset of hair bulb
• Hair Follicle Regeneration
• Exclusive Nutritive Blend

Hair loss is a problem that affects both men and women and has diverse origins, but despite the different etiologies, there is a common link between them: dermal papilla cells become latent, and all the hair growth processes stop. The multiactive ingredient CAPILIA LONGA ^PPF made from Curcuma longa (Turmeric) callus culture offers a solution to the problem. Seeing how hair loss starts when Dermal Papilla Cells become inactive, the solution of the problem goes through their reactivation. CAPILIA LONGA ^PPF is able to produce an epigenetic reset of the hair bulb, reactivating the activity of these latent dermal papilla cells and promoting the hair follicle regeneration to create new hair.

DESCRIPTION

Curcuma longa is a tropical and subtropical plant characterized by the existence of very ramified, cylindrical and orange rhizome. These rhizomes are a modified root that acts as a storage and resistance organ. They grow endlessly and have excellent regenerative properties.

Rich in curcuminoids (mainly curcumin), Curcuma longa is the most studied plant in biomedicine, having a huge potential, with more than 400 pre-clinic assays described in the literature and more than 1000 publications, showing its multiple properties (antioxidant, anti-inflammatory, wound healing, antimicrobial, DNA protection, etc...)

CAPILIA LONGA ^PPF represents a new activity profile for Curcuma longa, claiming the Phyto-Peptidic Fractions as a new range of bioactives from the species (Curcumin is only the 2-5% of turmeric powder). The ingredient is the concentrated secretome of totipotent cells from the rhizome (root) of Turmeric. This secretome is rich in signaling peptides specially designed to create the optimal micro-environment to re-activate hair growth, achieving an epigenetic reset of hair bulb and hair follicle regeneration.

CAPILIA LONGA ^PPF acts as a Hair 3D Bio-Printer, resetting and guiding the hair follicle growth with a bio-software (Phyto-Peptidic Frations), nourishing at the same time the hair follicle growth with an innovative Bio-Ink (Exclusive Nutritive Blend)

A GREAT TECHNOLOGY BEHIND

As expert developer of plant totipotent cell cultures, Vytrus Biotech launches a new range of products arising from the proprietary Plant Cell Biofactories Technology Platform, introducing the Phyto-Peptidic Fractions (PPF). They represent the first generation of genuine plant peptides, a revolutionary discovery that opens the door to a new generation of cosmetics.

Vytrus Biotech, through a driven Growth Factor Control development process has been able to identify, produce and concentrate these Phyto-Peptidic Fractions from an optimized culture of totipotent cells.

These PPF are the ultimate responsible of the extremely high regenerative properties of totipotent cells and, for the first time, this astonishing potential can be used in cosmetics

That makes of Phyto-Peptidic Fractions the first cosmetic active of this category able to maintain the skin regenerative potential.

IN VITRO EFFICACY

(To measure ability of ingredient to activate proliferation and release of key growth factors)

1. Induction of Dermal Papilla cells Proliferation.

• Human Fibroblasts Dermal Papilla Cells (HFDPC)
• 48 hours of incubation with the product
• Measurement of incorporation of Bromodeoxiuridine (BrdU) in the DNA of HFDPC through an Immunocto-chemical assay
• The levels of fluorescence intensity indicate the Proliferation Index of HFDPC compared to Not treated Cells in Basal Medium.

The results show up to 177% of Proliferation Index (compared to Not Treated cells), showing a Higher effect that other Growth Factors (HGF, FGF, VEGF, IGF-1) and even higher than Minoxidil (169%).

2. Induction of Insulin Growth Factor (IGF-1) Production from Dermal Papilla Cells

• Human Fibroblasts Dermal Papilla Cells (HFDPC)
• 24-48h of incubation with the product
• Quantification of IGF-1 levels from the supernatants by ELISA
• Production Index results compared to Not treated Cells in Basal Medium

Up to 115% of Production Index (compared to Not Treated cells) from the HFDPC, showing a faster activity than Minoxidil (36%).

3. Modulation of miRNA22 and miRNA33 expression levels from Derma Papilla Cells

• Human Fibroblasts Dermal Papilla Cells (HFDPC)
• 24h of incubation with the product
• Quantification of miRNA levels from HFDPC supernatants through qPCR
• Analysis of raw data by PfaffI method where the expression of each gene is compared with a control gen Actin (housekeeping gene)
• Production Index results compared to Not treated Cells in Basal Medium

Up to 6,3 times miRNA-31 Expression Stimulation (compared to Control)

Up to 40% miRNA-22 Expression Index Reduction (compared to Control)

IN VIVO EFFICACY

Panel of 40 volunteers

• Showing Hair Loss (A/T<4)
• Different etiologies*
• Men: grade Hamilton II-IV
• Women: grade Ludwig I-II
• 18-60 years old
• 1% dosage
• 1 application/day on the scalp
• Double blind assay. 45 - 90 - 150 days.

* Male and Female androgenetic alopecia, seasonal alopecia, diffuse alopecia, Hair loss due to technical processes, fragility, menopause.

Measurement of:

• Hair loss reduction (combing test)
• Hair density increase (general aspect and TrichoScan microphotographies)
• Rebalancing the hair growth cycle (Trichogram A/T analysis)

The results show:

Up to 89 % hair loss reduction after 150 days (average: 57 %)

Up to 52 % hair density increase after 150 days (average: 17 %, i.e. 13500 new hairs)

27 % increase of A/T ratio after 150 days

Improvement of hair anchoring and general hair strengthening.

Formulation: Water and ethanol soluble. Incorporation during the cooling phase (<40ºC

Dossage: Suggested use level: 0,5 -2%

Declarations: Free of GMO, BSE, listed CMR, VOC, heavy materials, listed allergens, parabens, phenoxyethanol, aflatoxins, pesticides and contaminant.

INCI: Curcuma Longa (Turmeric) Callus Conditioned Media

SAFETY PROFILE